NAT8L,N-乙酰转移酶8样蛋白抗体

产品编号HZ-11593R
英文名称NAT8L
中文名称N-乙酰转移酶8样蛋白抗体
别 名Camello-like protein 3; CML3; Hcml3; N acetyltransferase 8 like (GCN5 related, putative); N-acetylaspartate synthetase; N-acetyltransferase 8-like protein; NAA synthetase; NAT8 like; Nat8l; NAT8L_HUMAN.
说 明 书0.1ml 0.2ml
研究领域肿瘤 发育生物学 信号转导 细胞周期蛋白 细胞膜蛋白
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Pig, Horse, Rabbit,
NAT8L,N-乙酰转移酶8样蛋白抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量33kDa
细胞定位细胞浆 细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human NAT8L (201-302aa)
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
NAT8L,N-乙酰转移酶8样蛋白抗体PubMedPubMed
产品介绍background:
This gene encodes a single-pass membrane protein, which contains a conserved sequence of the GCN5 or NAT superfamily of N-acetyltransferases and is a member of the N-acyltransferase (NAT) superfamily. This protein is a neuron-specific protein and is the N-acetylaspartate (NAA) biosynthetic enzyme, catalyzing the NAA synthesis from L-aspartate and acetyl-CoA. NAA is a major storage and transport form of acetyl coenzyme A specific to the nervous system. The gene mutation results in primary NAA deficiency (hypoacetylaspartia).

Function:
Plays a role in the regulation of lipogenesis by producing N-acetylaspartate acid (NAA), a brain-specific metabolite. NAA occurs in high concentration in brain and its hydrolysis plays a significant part in the maintenance of intact white matter. Promotes dopamine uptake by regulating TNF-alpha expression. Attenuates methamphetamine-induced inhibition of dopamine uptake.

Subcellular Location:
Cytoplasm. Membrane; Single-pass membrane protein (Potential). Microsome membrane; Single-pass membrane protein (By similarity). Mitochondrion membrane; Single-pass membrane protein. Rough endoplasmic reticulum membrane; Single-pass membrane protein (By similarity). Note=Its enzymatic activity contribution is quantitatively larger in mitochondrial compartment than in extramitochondrial compartment.

Tissue Specificity:
Expressed in brain.

DISEASE:
Defects in NAT8L are the cause of N-acetylaspartate deficiency (NACED) [MIM:614063]. A metabolic disorder resulting in truncal ataxia, marked developmental delay, seizures, and secondary microcephaly.

Similarity:
Belongs to the camello family.
Contains 1 N-acetyltransferase domain.

Database links:
UniProtKB/Swiss-Prot: Q8N9F0.3

NAT8L,N-乙酰转移酶8样蛋白抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Tissue/cell: human stomach carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-NAT8L Polyclonal Antibody, Unconjugated(HZ-11593R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining