DNA试剂盒
产品名称: DNA试剂盒
英文名称: Quick Blunting Kit
产品编号: E1201L
产品价格: 0
产品产地: 美国
品牌商标: NEB
更新时间: null
使用范围: null
北京百奥创新科技有限公司
- 联系人 :
- 地址 : 北京市海淀区创客小镇
- 邮编 :
- 所在区域 : 北京
- 电话 : 133****0421 点击查看
- 传真 : 点击查看
- 邮箱 : customer@bestopbio.com;3007606172@qq.com
DNA is blunted using T4 DNA Polymerase (NEB #M0203) which has both 3 → 5 exonuclease activity and 5 → 3 polymerase activity. T4 Polynucleotide Kinase (NEB #M0201) is included in the enzyme mix for phosphorylation of the 5 ends of blunt-ended DNA for subsequent ligation into a cloning vector. This kit is optimized for blunting up to 5 g of DNA in a single reaction. Blunt Enzyme Mix supplied in: 100 mM KCl, 10 mM Tris-HCl(pH 7.4), 0.1 mM EDTA, 1 mM dithiothreitol, 0.1% Triton X-100 and 50% Glycerol.