PCDHA8,原钙粘附蛋白α8抗体

产品编号HZ-11122R
英文名称PCDHA8
中文名称原钙粘附蛋白α8抗体
别 名KIAA0345 like 6; PCDH ALPHA8; Protocadherin alpha 8; PCDA8_HUMAN; PCDH alpha 8; PCDH-alpha-8; Protocadherin alpha 8; Protocadherin alpha-8.
说 明 书0.1ml 0.2ml
研究领域神经生物学 信号转导 细胞粘附分子 细胞骨架 细胞膜蛋白
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep,
PCDHA8,原钙粘附蛋白α8抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量100kDa
细胞定位细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human PCDHA8
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PCDHA8,原钙粘附蛋白α8抗体PubMedPubMed
产品介绍background:
Protocadherins are a large family of cadherin-like cell adhesion proteins that are involved in the establishment and maintenance of neuronal connections in the brain. There are three protocadherin gene clusters, designated alpha, beta and gamma, all of which contain multiple tandemly arranged genes. PCDHA8 (protocadherin alpha 8) is a 950 amino acid single-pass type I membrane protein that contains six cadherin domains and is encoded by a gene which is located within the protocadherin alpha gene cluster on human chromosome 5. Existing as multiple alternatively spliced isoforms, PCDHA8 functions as a potential calcium-dependent cell adhesion protein that may be involved in the establishment and maintenance of neuronal connections within the brain.

Function:
PCDHA8 is most likely to play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been observed and additional variants have been suggested but their full-length nature has yet to be determined. Two different isoforms are known.

Subcellular Location:
Cell membrane; Single-pass type I membrane protein

Similarity:
Contains 6 cadherin domains.

Database links:
UniProtKB/Swiss-Prot: Q9Y5H6.1

PCDHA8,原钙粘附蛋白α8抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Protocadherin alpha 8; PCDHA8) Polyclonal Antibody, Unconjugated (HZ-11122R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Protocadherin alpha 8; PCDHA8) Polyclonal Antibody, Unconjugated (HZ-11122R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.