APPBP1,β淀粉样蛋白前体蛋白结合蛋白1抗体

产品编号HZ-12503R
英文名称APPBP1
中文名称β淀粉样蛋白前体蛋白结合蛋白1抗体
别 名59 kDa; A 116A10.1; Amyloid beta precursor protein binding protein 1 59kD; Amyloid beta precursor protein binding protein 1 59kDa; Amyloid beta precursor protein binding protein 1; Amyloid beta precursor protein-binding protein 1; Amyloid protein binding protein 1; Amyloid protein-binding protein 1; APP BP1; APP-BP1; APPBP 1; HPP 1; HPP1; NAE 1; NAE1; NEDD8 activating enzyme E1 regulatory subunit; NEDD8 activating enzyme E1 subunit 1; NEDD8 activating enzyme E1 subunit; NEDD8-activating enzyme E1 regulatory subunit; Proto oncogene protein 1; Proto-oncogene protein 1; Protooncogene protein 1; ula 1; ULA1_HUMAN.
说 明 书0.1ml 0.2ml
研究领域细胞生物 神经生物学 表观遗传学 泛素 细胞膜蛋白 Alzheimer's
抗体来源Rabbit
APPBP1,β淀粉样蛋白前体蛋白结合蛋白1抗体克隆类型Polyclonal
交叉反应Human, Mouse, Rat, Dog, Pig, Horse, Rabbit,
产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量60kDa
细胞定位细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human APPBP1
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
APPBP1,β淀粉样蛋白前体蛋白结合蛋白1抗体PubMedPubMed
产品介绍background:
APPBP1 is a member of the ubiquitin-activating E1 family. In fetal tissues APPBP1 is widely expressed and in adult tissues it is expressed throughout the brain. APPBP1 is a cell membrane associated protein and functions as the regulatory subunit in a heterodimer with UBA3. The APPBP1/UBA3 complex binds to and activates NEDD8, a ubiquitin-like protein involved in signal transduction, cell proliferation and development. This suggests that APPBP1 affects a variety of cellular functions. In addition, APPBP1 is essential for cell cycle progression through the S/M checkpoint. More specifically, it inhibits the entry into S phase and promotes entry into M phase.

Function:
Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation.

Subunit:
Heterodimer of UBA3 and NAE1. The complex binds NEDD8 and UBE2M. Binds APP and TP53BP2.

Subcellular Location:
Cell membrane. Colocalizes with APP in lipid rafts.

Tissue Specificity:
Ubiquitous in fetal tissues. Expressed throughout the adult brain.

Post-translational modifications:
Ubiquitinated by TRIP12, leading to its degradation by the proteasome.

APPBP1,β淀粉样蛋白前体蛋白结合蛋白1抗体Similarity:
Belongs to the ubiquitin-activating E1 family. ULA1 subfamily.

Database links:
Entrez Gene: 8883 Human
Entrez Gene: 234664 Mouse
Omim: 603385 Human
SwissProt: Q13564 Human
SwissProt: Q8VBW6 Mouse
Unigene: 460978 Human
Unigene: 237670 Mouse

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.