FITC标记的金属蛋白酶组织抑制因子-4抗体
产品名称： FITC标记的金属蛋白酶组织抑制因子-4抗体
英文名称： Anti-TIMP-4/FITC
产品编号： HZ-0418R-FITC
产品价格： null
产品产地： 中国/上海
品牌商标： HZbscience
更新时间： 2023-08-17T10:24:20
使用范围： IF=1:50-200
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Rabbit Anti-TIMP-4/FITC Conjugated antibody
FITC标记的金属蛋白酶组织抑制因子-4抗体
| 英文名称 | Anti-TIMP-4/FITC |
| 中文名称 | FITC标记的金属蛋白酶组织抑制因子-4抗体 |
| 别 名 | Metalloproteinase inhibitor 4; TIMP 4; TIMP4; TIMP metallopeptidase inhibitor 4; TIMP-4; Timp4; TIMP4_HUMAN; Tissue inhibitor of metalloproteinase 4. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 肿瘤 心血管 神经生物学 合成与降解 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Cow, Horse, Rabbit, |
| 产品应用 | IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 22kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TIMP-4 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: The tissue inhibitors of metalloproteinases (TIMPs) are naturally occurring proteins that specifically inhibit matrix metalloproteinases and regulate extracellular matrix turnover and tissue remodeling by forming tightbinding inhibitory complexes with the MMPs. Thus, TIMPs maintain the balance between matrix destruction and formation. An imbalance between MMPs and the associated TIMPs may play a significant role in the invasive phenotype of malignant tumors. The TIMP proteins share several structural features including six loops held in place by six disulfide bonds arranged in three knot-like structures. These proteins also contain twelve cysteine residues in conserved regions of the molecule that form six disulfide bonds, essential for the formation of native conformations, and the N terminal region that is necessary for inhibitory activities. The N terminus of each TIMP contains a consensus sequence (VIRAK) and each TIMP is translated with a 29 amino acid leader sequence that is cleaved off to produce the mature protein. The C terminal regions are divergent, which enhances the selectivity of inhibition and binding efficiency. Although the TIMP proteins share high homology, they may either be secreted extracellularly in soluble form (TIMP1, TIMP2 and TIMP4) or bind to extracellular matrix components (TIMP3). The MMPs and TIMPs can be divided into two groups with respect to gene expression: the majority exhibit inducible expression and a small number are produced constitutively or are expressed at very low levels and are not inducible. Among agents that induce MMP and TIMP production are the inflammatory cytokines TNF alpha and IL1 beta. A marked cell type specificity is a hallmark of both MMP and TIMP gene expression (i.e. a limited number of cell types can be induced to make these proteins). Tissue Inhibitor of Metalloproteinases 4 (TIMP4) was identified by molecular cloning. TIMP4 shows 37 % amino acid identity with TIMP1 and 51 % homology with TIMP2 and TIMP3. TIMP4 is secreted extracellularly, predominantly in heart and brain tissue. It may function in a tissue specific fashion in extracellular matrix (ECM) homeostasis. TIMP4 has a strong inhibitory effect on the invasion of human breast cancer cells across reconstituted basement membranes suggesting that TIMP4 may have an important role in inhibiting primary tumor growth and progression. The human TIMP4 gene has the chromosomal location of 3p25. Function: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7 and MMP-9. Subcellular Location: Secreted. Tissue Specificity: Abundant in heart and present at low levels in many other tissues. Similarity: Belongs to the protease inhibitor I35 (TIMP) family. Contains 1 NTR domain. Database links: Entrez Gene: 7079 Human Entrez Gene: 110595 Mouse Entrez Gene: 114849 Rat Entrez Gene: 680130 Rat Omim: 601915 Human SwissProt: Q99727 Human SwissProt: Q9JHB3 Mouse SwissProt: P81556 Rat Unigene: 591665 Human Unigene: 255607 Mouse Unigene: 155651 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 合成与降解（Synthesis and Degradation） TIMP-4是TIMP家族的新成员，TIMP-3与TIMP-4同样具有较强的促凋亡、抑制血管生成、抗肿瘤浸润作用 |
金属蛋白酶组织抑制剂(TIMPs)是天然存在的蛋白质，通过与基质金属蛋白酶形成紧密结合的抑制复合物，特异性抑制基质金属蛋白酶，调节细胞外基质周转和组织重塑。因此，TIMPs保持了矩阵破坏和形成之间的平衡。MMPs和相关TIMP之间的不平衡可能在恶性肿瘤的侵袭表型中发挥重要作用。TIMP蛋白具有若干结构特征，包括由六个二硫键在适当位置保持的六个环，这些二硫键排列成三个结状结构。这些蛋白质还含有十二个半胱氨酸残基在分子的保守区域，形成六个二硫键，对于形成天然构象是必不可少的，以及抑制活性所必需的N端区域。每个TIMP的N端包含一个一致序列（VIRAK），并且每个TIMP被翻译成29个氨基酸前导序列，该序列被切割出来产生成熟蛋白。C末端区域是发散的，增强了抑制和结合效率的选择性。尽管TIMP蛋白具有高度同源性，但它们可以以以可溶性形式(TIMP1、TIMP2和TIMP4)在细胞外分泌，或者与细胞外基质成分(TIMP3)结合。
MMP和TIMP在基因表达方面可分为两组：大多数表达可诱导，少数组成性地产生或表达在非常低的水平，而不可诱导。诱导MMP和TIMP产生的药物是炎性细胞因子TNF-α和IL1β。显著的细胞类型特异性是MMP和TIMP基因表达的标志(即，可以诱导有限数量的细胞类型来制造这些蛋白)。通过分子克隆鉴定金属蛋白酶组织抑制因子4（TIMP4）。TIMP4与TIMP1具有37%的氨基酸同源性，与TIMP2和TIMP3同源性为51%。TIMP4在细胞外分泌，主要分布于心脏和脑组织。它可能以组织特异性方式在细胞外基质（ECM）稳态中发挥作用。TIMP4对人乳腺癌细胞跨重组基底膜的侵袭有强烈的抑制作用，提示TIMP4在抑制原发性肿瘤生长和进展方面可能具有重要作用。人类TIMP4基因的染色体位置为3p25。