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上海信裕生物科技有限公司
MSH2抗体

MSH2抗体

商家询价

产品名称： MSH2抗体

英文名称： MSH2Antibody

产品编号： XY15520-1-

产品价格： null

产品产地： 中国/美国

品牌商标： XYbscience

更新时间： 2023-08-17T09:55:27

使用范围： WB,ELISA,IHC-P,IHC-F,IF

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 MSH2抗体

Applications
In-house applications ELISA, WB, IHC, IF, FC, IP     Application not tested? Please see our Guarantee.
Published applications IHC, WB Publications 5 citations –view here
Species specificity Human,Mouse,Rat; other species not tested.
Published Species Human
Images
Prev12NextView more validation data
  • 15520-1-AP;HeLa cellsHeLa cells were subjected to SDS PAGE followed by western blot with 15520-1-AP(MSH2 antibody) at dilution of 1:500
  • 15520-1-AP;human colon cancerImmunohistochemical of paraffin-embedded human colon cancer using 15520-1-AP(MSH2 antibody) at dilution of 1:100 (under 10x lens)
  • 15520-1-AP;human colon cancerImmunohistochemical of paraffin-embedded human colon cancer using 15520-1-AP(MSH2 antibody) at dilution of 1:100 (under 40x lens)
  • 15520-1-AP;HelaImmunofluorescent analysis of Hela cells, using MSH2 antibody 15520-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
  • 15520-1-AP;HeLa cellsIP Result of anti-MSH2 (IP:15520-1-AP, 5ug; Detection:15520-1-AP 1:500) with HeLa cells lysate 2000ug.
  • 15520-1-AP;HeLa cells1X10^6 HeLa cells were stained with 0.2ug MSH2 antibody (15520-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
Positive WB detected in HeLa cells, A2780 cells
Positive IP detected in HeLa cells
Positive IHC detected in Human colon cancer tissue, human tonsil tissue
Positive IF detected in Hela cells
Positive FC detected in HeLa cells
Recommended dilution WB : 1:1000-1:10000
IP : 1:200-1:2000
IHC : 1:20-1:200
IF : 1:10-1:100
 MSH2抗体Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Product Information
Source Rabbit Purification method Antigen affinity purification
Isotype IgG Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC.
Immunogen Information MSH2抗体
Immunogen MSH2 fusion protein ag7835 Full name MutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli)
Calculated molecular
weight		 105 kDa Observed molecular 
weight		 100 kDa-105 kDa
GenBank accession 
number		 BC021566 Gene ID (NCBI) 4436
Gene symbol MSH2 Synonyms COCA1, FCC1, hMSH2, HNPCC, HNPCC1, LCFS2, MSH2, MutS protein homolog 2
Background

MSH2, named for its homologous to the E.coli MutS gene, takes part in DNA mismatch repair(MMR) to maintain the stability of DNA. MSH2 contains a helix-turn-helix domain, which responds for binding to DNA. Also, MSH2 has a direct role in mutation avoidance and micro-satellite stability in cell. MSH2抗体

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