P2RX4,三磷酸腺苷门控阳离子通道蛋白抗体

产品编号HZ-7690R
英文名称P2RX4
中文名称三磷酸腺苷门控阳离子通道蛋白抗体
别 名ATP gated cation channel protein; ATP receptor; p2rx4; P2RX4_HUMAN; P2X purinoceptor 4; P2X receptor subunit 4; P2X4; P2X4R; Purinergic receptor; Purinergic receptor P2X ligand gated ion channel 4; Purinergic receptor P2X4; Purinoceptor P2X4.
说 明 书0.1ml 0.2ml
研究领域肿瘤 心血管 细胞生物 神经生物学 信号转导 通道蛋白
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Cow,
P2RX4,三磷酸腺苷门控阳离子通道蛋白抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量43kDa
细胞定位细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human P2RX4
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
P2RX4,三磷酸腺苷门控阳离子通道蛋白抗体PubMedPubMed
产品介绍background:
The P2X receptor family is comprised of ligand-gated ion channels that allow for the increased permeability of calcium into the cell in response to extracellular ATP. The seven P2X receptors, P2X1-P2X7, form either homomeric or heteromeric channels or both. They are characterized by intracellular amino- and carboxy-termini. P2X receptors are expressed in a wide variety of tissues, including neurons, prostate, bladder, pancreas, colon, testis and ovary. The major function of the P2X receptors is to mediate synaptic transmissions between neurons and to other tissues via the binding of extracellular ATP, which acts as a neurotransmitter. The P2X receptors may be involved in the onset of necrosis or apoptosis after prolonged exposure to high concentrations of extracellular ATP.

Function:
Receptor for ATP that acts as a ligand-gated ion channel. This receptor is insensitive to the antagonists PPADS and suramin.

Subunit:
Functional P2XRs are organized as homomeric and heteromeric trimers.

Subcellular Location:
Membrane; Multi-pass membrane protein.

P2RX4,三磷酸腺苷门控阳离子通道蛋白抗体Similarity:
Belongs to the P2X receptor family.

Gene ID:
5025

Database links:
Entrez Gene: 338036 Cow
Entrez Gene: 448783 Dog
Entrez Gene: 5025 Human
Entrez Gene: 18438 Mouse
Entrez Gene: 100458831 Orangutan
Entrez Gene: 29659 Rat
Omim: 600846 Human
SwissProt: Q5E9U1 Cow
SwissProt: Q99571 Human
SwissProt: Q9JJX6 Mouse
SwissProt: P51577 Rat
Unigene: 321709 Human
Unigene: 290884 Mouse
Unigene: 463383 Mouse
Unigene: 7176 Rat

P2RX4,三磷酸腺苷门控阳离子通道蛋白抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Tissue/cell: mouse colon tissue;4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min;  Incubation: Anti-P2RX4 Polyclonal Antibody, Unconjugated(HZ-7690R) 1:200, overnight at 4∑C; The secondary antibody was Goat Anti-Rabbit IgG, FITC conjugated(HZ-0295G-FITC)used at 1:200 dilution for 40 minutes at 37∑C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min;  Incubation: Anti-P2RX4 Polyclonal Antibody, Unconjugated(HZ-7690R) 1:200, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Paraformaldehyde-fixed, paraffin embedded (Rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ATP gated cation channel protein; ATP receptor; p2rx4; P2X4/P2RX4) Polyclonal Antibody, Unconjugated (HZ-7690R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining. Tissue/cell: rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min;  Incubation: Anti-P2RX4 Polyclonal Antibody, Unconjugated(HZ-7690R) 1:200, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining