Anti-DR5 mAb, clone DR5-01
Cat. No.: MC-897 (100 μg)
Specificity:
Apoptosis, or programmed cell death, occurs
during normal cellular differentiation and
development of multicellular organisms.
Apoptosis is induced by certain cytokines
including Tumor Necrosis Factor (TNF) and Fas
Ligand in the TNF family through their death
domain containing receptors, TNF Receptor 1
(TNFR1) and Fas, respectively. Another
member in the TNF family has been identified
and designated TRAIL (TNF Related Apoptosis
Inducing Ligand) and Apo2L (Apo2 Ligand).
Receptors for TRAIL include two death domain
containing receptors, DR4 and DR5, as well as
two decoy receptors, DcR1 and DcR2, lacking
the intracellular signaling death domain. DcR1
(TRID), like the related death receptors DR4 and
DR5, contains two extracellular cysteine rich
domains. However, DcR1 contains no intracellular
death domain and is thus incapable of
signaling apoptosis. It has been suggested that
DcR1 is responsible for TRAIL resistance in
normal human tissues including heart, placenta,
lung, liver, kidney, spleen, and bone marrow.
DR5 is a member of the TNF receptor
superfamily, and contains an intracellular death
domain. This receptor can be activated by
Tumor Necrosis Factor related apoptosis
including ligand (TNFSF10/TRAIL/APO2L), and
tranduces apoptosis signal. Studies with FADD
deficient mice suggested that FADD, a death
domain containing adaptor protein, is required
for the apoptosis mediated by this protein.
Immunogen:
Recombinant fusion protein of human IgG heavy
chain and extracellular domain of DR5.
Cellular Localization:
This antibody recognizes the extracellular
domain of DR5.
Ig Isotype:
Mouse IgG1
Species Reactivity:
Human. Others not tested.
Format:
100 μg of monoclonal antibody purified from
hybridoma culture supernatant at approxiamtely
1 mg/mL in PBS containing 15 mM sodium azide,
pH 7.4.
Storage:
Store at 4°C short term, -20°C long term.
Aliquot to avoid freeze/thaw cycles.
Applications:

Flow cytometry
The optimal dilution for a specific application
should be determined by the researcher.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product.