小鼠磷酸烯醇式丙酮酸羧激酶1(PCK1)多克隆抗体
Polyclonal Antibody to Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1)
[PROPERTIES]
Source: Polyclonal antibody preparation
Host: Rabbit
Purification: Antigen-specific Affinity Chromatography. Traits: Liquid
Concentration: 200µg/mL
UOM: 100µg
Applications: WB; IHC; ICC; IP.
[ IMMUNOGEN ]
Immunogen: Recombinant PCK1 (Ile312~Ala563) expressed in E.coli. 
[ APPLICATIONS ]
Western blotting: 0.5-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.
[ FORMULATION ]
Form & Buffer: Supplied as solution form in 0.01M PBS, pH7.4, containing
0.05% Proclin-300, 50% glycerol.
[STORAGE AND STABILITY] 
Storage: Avoid repeated freeze/thaw cycles.
Store at 4ºC for frequent use.
Aliquot and store at -20ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determinedby accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and noobvious degradation and precipitation were observed. The loss rate is less than 5% within theexpiration date under appropriate storage condition.
[IDENTIFICATION]

Figure 1. Western Blot
A. Sample: Recombinant PCK1, Mouse B. Lane1: Mouse Liver Tissue Lane2: Rat Liver Tissue Lane3: Mouse Kidney Tissue Primary Ab: 2µg/mL Rabbit Anti-Mouse PCK1 Ab
Second Ab: 1:2000 Dilution of HRP-Linked Guinea pig
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