Recombinant  Human Thymosin-β4
Catalog  Number:
140-14
Synonyms:
Tβ-4,  Hematopoietic system regulatory peptide, Seraspenide, TMSB4X, TB4X, TMSB4
Description:
Thymosin-β4  is a small, actin-sequestering protein belonging to the thymosin-β family that  is found at high concentrations within the spleen, thymus, and peritoneal  macrophages, where it is most notably responsible for the organization of  cytoskeletal structure. In mammalian tissues, this protein acts as a modulator  for the polymerization/depolymerization of actin through the formation of a 1:1  complex with the monomer G (globular)-actin, and inhibits actin’s polymerization  to form F (filamentous) actin, which together with other proteins binds  microfilaments to construct the cytoskeleton. Commonly found at significant  quantities within the brain, lungs, liver, kidneys, testes, and heart,  Thymosin-β4 has also been shown to be synthesized by cells unrelated to the  reticuloendothelial system, such as myoblasts and fibroblasts, and expressed at  irregular levels by several hemopoietic cell lines, malignant lymphoid cells and  myeloma cells. In addition to regulating actin polymerization, research has also  found Thymosin-β4 to stimulate the secretion of hypothalamic luteinizing  hormone-releasing hormone and luteinizing hormone, inhibit the migration of  peritoneal macrophages, induce phenotypic changes in T cell lines during early  host defense mechanisms, and inhibit the progression of hematopoietic  pluripotent stem cells into the s-phase. Recombinant Human Thymosin-β4 is a 5.2  kDa glycoprotein containing 45 amino acid residues.
AA  Sequence:
RMSDKPDMAE IEKFDKSKLK KTETQEKNPL PSKETIEQEK QAGES
Source:
E.coli
Purity:
Greater  than 95% by SDS-PAGE gel and HPLC analyses.
Biological  Activity:
Pretreatment  of primary lung fibroblasts with recombinant Thymosin-β4, using a concentration  of 0.5 - 10 μg/ml, produces a protective effect against hydrogen peroxide  induced cell death.
 
 
 
 
Research  Interests:
 
 
 
 
Apoptosis
 
 
Cancer
 
 
 
Stem  Cells & Differentiation
 
 
 
 
Country  of Origin:
USA
Research  Use Only. Not for use in diagnostic or therapeutic procedures.