Product: Anti-human Chondroitin/Keratan Sulfate Proteoglycan mAb, clone 5C5
Cat. No.: MC-797 (100 μL)
Specificity:
Reacts with chondroitin sulfate proteoglycan
from cartilage (aggrecan).
Species Reactivity:
Reacts with human. No cross-reactivity with
bovine nasal cartilage, rat chondrosarcoma,
chick or shark cartilage aggrecan. Other
species not tested.
Ig Isotype: mouse IgG1
Immunogen:
Human large molecular weight proteoglycan of
articular cartilage (aggrecan)
Format:
100 μL ascites containing 0.01% sodium azide.
Antibody concentration >1 mg/mL. Purified by
Ascites fluid.
Storage and Stability:
-20°C long term, 4°C short term. Stable for at
least 24 months when stored properly.
Applications and Suggested Dilutions:

ELISA: 1:500-1:1,000

Immunoprecipitation: 1:50-1:300

Immunohistochemistry: (paraffin embedded):
1:50-1:3,000. Treat tissue sections with 2
mg/ml testicular hyaluronidase in Na-acetate
buffer, pH 5.5 for 1 hr at 37C, followed by
treatment with 0.2 mg/ml Protease XXIV in
TBS, pH 7.2, for the same time and
temperature.

Western blotting: 1:100-1:300
The optimal dilution for a specific application
should be determined by the researcher.
References:
1. Vertel (1995) Trends Cell Biology 5: 458-464.
2. Iozzo (1998) Annual Reviews in Biochemistry
67: 609-52.
3. Schwartz et al. (1999) Progress in Nucleic
Acid Research and Molecular Biology 62:
177-225.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product.