FOXG1,叉头蛋白G1抗体

产品编号HZ-11557R
英文名称FOXG1
中文名称叉头蛋白G1抗体
别 名BF 1; BF 2; BF-1; BF-2; BF1; BF2 ; Brain factor 1; Brain factor 2; FHKL; FKH2; FKHL1; FKHL2; FKHL2; FKHL3; FKHL4; Forkhead box G1A; Forkhead box G1B; Forkhead box protein G1; Forkhead box protein G1A; Forkhead box protein G1B; Forkhead box protein G1C; Forkhead drosophila homolog like 2; Forkhead like 1; Forkhead like 2; FOXG1_HUMAN.
说 明 书0.1ml 0.2ml
研究领域细胞生物 神经生物学 信号转导 转录调节因子 表观遗传学
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Horse, Rabbit,
FOXG1,叉头蛋白G1抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量52kDa
细胞定位细胞核
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human FOXG1 (201-300aa)
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
FOXG1,叉头蛋白G1抗体PubMedPubMed
产品介绍background:
The winged-helix transcriptional repressor (WH) BF-1 gene encodes brain factor 1 (BF-1), also known as foxg1, and is essential for the proliferation of progenitor cells in the cerebral cortex and influences regional patterning in the mammalian telencephalon (1–4). WH proteins are a family of putative transcriptional regulators with diverse roles in development, and are characterized by a highly conserved DNA binding structure, the WH domain (1,5,6). BF-1 plays a critical role in the development of the cerebral hemispheres of the brain and targeted disruption of the gene leads to severe defects in the development of telencephalic structures, such as the cerebral cortex and basal ganglia (1). The loss of BF-1 results in an accelerated rate of neuronal differentiation and the shortening of the neurogenetic period in the embryonic cerebral cortex (1,7). BF-1 is expressed by E8.5 in telencephalic progenitors (1). It may also regulate the response of cerebral cortical progenitors to environmental cues (1).

Function:
Transcription repression factor which plays an important role in the establishment of the regional subdivision of the developing brain and in the development of the telencephalon.

Subunit:
Interacts with KDM5B.

Subcellular Location:
Nucleus.

Tissue Specificity:
Expression is restricted to the neurons of the developing telencephalon.

DISEASE:
Defects in FOXG1 are the cause of congenital variant of Rett syndrome (RTTCV) [MIM:613454]. RTTCV is a severe neurodevelopmental disorder with features of classic Rett syndrome but earlier onset in the first months of life. Clinical features include progressive microcephaly, hypotonia, irresponsiveness and irritability in the neonatal period, mental retardation, psychomotor regression and stereotypical movements.

FOXG1,叉头蛋白G1抗体Similarity:
Contains 1 fork-head DNA-binding domain.

Gene ID:
2290

Database links:
Entrez Gene: 2290 Human
Entrez Gene: 15228 Mouse
Omim: 164874 Human
SwissProt: P55316 Human
SwissProt: Q60987 Mouse
Unigene: 695962 Human
Unigene: 708841 Human
Unigene: 4704 Mouse

FOXG1,叉头蛋白G1抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Forkhead drosophila homolog like 2) Polyclonal Antibody, Unconjugated (HZ-11557R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.