GRP78/BIP抗体-抗体-抗体-生物在线
上海信裕生物科技有限公司
GRP78/BIP抗体

GRP78/BIP抗体

商家询价

产品名称： GRP78/BIP抗体

英文名称： GRP78/BIPAntibody

产品编号： XY11587-1

产品价格： null

产品产地： 中国/美国

品牌商标： XYbscience

更新时间： 2023-08-17T09:55:27

使用范围： WB,ELISA,IHC-P,IHC-F,IF

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 GRP78/BIP抗体

'Applications

In-house applications ELISA, WB, IP, IF, FC, IHC     Application not tested? Please see our Guarantee.
Published applications IF, IHC, IP, WB Publications 29 citations –view here
Species specificity Human, Mouse, Rat; other species not tested.
Published Species Baby Hamster Syrian, human, mouse, rat, zebrafish
Images
Prev12NextView more validation data
  • 11587-1-AP;HepG2 cellsHepG2 cells were subjected to SDS PAGE followed by western blot with 11587-1-AP(GRP78,BIP antibody) at dilution of 1:1000
  • 11587-1-AP;human pancreas cancer tissueImmunohistochemical of paraffin-embedded human pancreas cancer using 11587-1-AP(GRP78,BIP antibody) at dilution of 1:50 (under 10x lens)
  • 11587-1-AP;human pancreas cancer tissueImmunohistochemical of paraffin-embedded human pancreas cancer using 11587-1-AP(GRP78,BIP antibody) at dilution of 1:50 (under 40x lens)
  • 11587-1-AP;HepG2 cellsImmunofluorescent analysis of HepG2 cells using 11587-1-AP( GRP78,BIP Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG
  • 11587-1-AP;HeLa cells1X10^6 HeLa cells were stained with 0.2ug GRP78,BIP antibody (11587-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
  • 11587-1-AP;MCF-7 cellsIP Result of anti-GRP78,BIP (IP:11587-1-AP, 3ug; Detection:11587-1-AP 1:1000) with MCF-7 cells lysate 3250ug.
Positive WB detected in HepG2 cells, BxPC-3 cells, HeLa cells, MCF7 cells, rat liver tissue, TN treated Hela cells
Positive IP detected in MCF-7 cells
Positive IHC detected in Human pancreas cancer tissue, human brain tissue, human breast cancer tissue, human colon cancer tissue, mouse heart tissue
Positive IF detected in HepG2 cells
Positive FC detected in HeLa cells
Recommended dilution WB : 1:500-1:5000
IP : 1:500-1:5000
IHC : 1:20-1:200
IF : 1:10-1:100
 GRP78/BIP抗体Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Product Information
Source Rabbit Purification method Antigen affinity purification
Isotype IgG Storage PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC.
Immunogen Information
Immunogen GRP78/BIP fusion protein ag2188 Full name Heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
Calculated molecular
weight		 78 kDa Observed molecular 
weight		 78 kDa
GenBank accession 
number		 BC020235 Gene ID (NCBI) 3309
Gene symbol HSPA5 Synonyms BIP, GRP 78, GRP78, HSP A5, HSPA5, MIF2
Background GRP78/BIP抗体

GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER).It is a constitutively expressed resident protein of the ER in all eukaryotic cells. Recently it has been reported that GRP78 is associated with apoptosis or inhibition of cancer cell growth. GRP78/BIP抗体

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