产品性质
别名(Synonyms) | CD340; EGFR2; ErbB2; HER2; HER-2; herstatin; MLN 19; MLN19; NGL; TKR1; ERBB2; NEU; ENV; ENVW; ERVWE1; HERV7Q; HERVW |
Uniprot No. | |
表达区间及表达系统(Source) | Recombinant Human Her2/ErbB2 Protein is expressed from HEK293 Cells with hFc tag at the C-terminal. It contains Thr23-Thr652. |
分子量(Molecular Weight) | The protein has a predicted MW of 96.1 kDa. Due to glycosylation, the protein migrates to 100-120 kDa based on Tris-Bis PAGE result. |
外观(Physical Appearance) | Sterile Filtered White lyophilized (freeze-dried) powder. |
纯度(Purity) | > 95% as determined by SDS-PAGE and HPLC |
活性(Activity) | ELISA Data:Immobilized Human Her2, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-Her2 Antibody, hFc Tag with the EC50 of 86.1ng/ml determined by ELISA. |
内毒素(Endotoxin) | < 1.0 EU per 1μg of the protein by the LAL method. |
制剂(Formulation) | Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
复溶(Reconstitution) | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
运输与保存方法
冰袋运输。-20℃至-80℃保存,一年有效期。
复溶后,-20 至 -80°C,在未开封状态下保存 3-6 个月。复溶后,2-8°C 保存 2-7 天。
建议第一次使用时分装冻存,避免反复冻融。
注意事项
1. 避免反复冻融。
2. 为了您的安全和健康,请穿实验服并戴一次性手套操作。
3. 本产品仅作科研用途!
产品数据
Tris-Bis PAGE