分子别名(Synonym):PCSK9,FH3,HCHOLA3,LDLCQ1,NARC1,PC9
表达区间及表达系统(Source):Human PCSK9, His Tag (PC9-H5223) is expressed from human 293 cells (HEK293). It contains AA Gln 31 - Gln 692 (Accession # Q8NBP7-1 ).
Predicted N-terminus: Gln 31 & Ser 153
蛋白结构(Molecular Characterization):This protein carries a polyhistidine tag at the C-terminus. This protein undergoes autocatalytic cleavage to release the pro-peptide and mature chain. The pro-peptide and mature chain are associated through non‑covalent interactions and with a calculated MW of 13.8 kDa and 59.2 kDa respectively. The protein migrates as 17 kDa and 65-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素(Endotoxin):Less than 1.0 EU per μg by the LAL method.
纯度(Purity):>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂(Formulation):Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
存储(Storage):For long term storage, the product should be stored at lyophilized state at -20°C or lower.
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